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    [COVID-19] AccuPower Real-Time PCR KIT
    AccuPower® SARS-CoV-2 Real-Time RT-PCR Kit is an in vitro diagnostic kit that helps diagnose COVID-19(Coronavirus disease 2019). This kit is designed for the detection of SARS-CoV-2 (E gene and RdRp gene) RNA from a COVID-19 suspected patient’s sample through Real-Time Polymerase chain reaction (PCR


    Introduction 

    Real-Time PCR is a technology that allows real-time monitoring of the amount of amplification products by measuring the fluorescence intensity generated by amplifying a specific DNA sequence. The amplification of the PCR product is measured using primer and probe marked with fluorescent material, which is specific to the specific sequence that is amplified, thus indicating a high specificity. TaqMan® Probe, commonly used in Real-time PCRs, is labeled as a "quencher" at the end of 5' with the fluorescent material at the end of 3' so that when combined with template DNA, the energy of the fluorescence is transferred to the quencher and does not emit fluorescence.
    However, as the PCR process progresses, 5' - 3' exonuclease of DNA polymerase is activated and released fluorescence by separating it from the fluorescence probe. The fluorescence produced can be measured in real time and displayed as a PCR amplification curve at each cycle of the PCR, which enables detection of specific RNA in the specimen.
    AccuPower® SARS-CoV-2 Real-Time RT-PCR Kit is master mix type, containing primers, dual-labeled fluorogenic (TaqMan®) probe, DNA polymerase, dNTPs, stabilizer and so on.

     
    1. SARS-CoV-2 Detection: SARS-CoV-2 (E gene and RdRp gene) RNA can be detected with high sensitivity.
    2. Enhanced Sensitivity & Specificity: Bioneer’s proprietary Dual-HotStart technology accomplishes high sensitivity and specificity.
    3. High Quality: All diagnostic kits manufactured by Bioneer are under strict quality control guidelines.
    4. Compatible with various system: Exicycler™ 96(BIONEER), CFX 96(Bio-rad), ABI7500Fast(Thermofisher)
     
     Specimen Type  Sputum, Nasopharyngeal swab, Oropharyngeal swab
      Kit Contents  1. Master mix / 1,000ul/tube x 1ea
     2. Enzyme mix / 500ul/tube x 1ea
     3. Oligo mix 1 / 250ul/tube x 1ea
     4. Oligo mix 2 / 250ul/tube x 1ea
     5. Positive Control / 150ul/tube x 1ea
      Instrumentation  Exicycler™ 96, CFX96, ABI 7500 FAST
      Tests  50 tests